DAO 85:15-22 (2009)  -  DOI: https://doi.org/10.3354/dao02051

Primary diagnosis and surveillance of white spot syndrome virus in wild and farmed crawfish (Procambarus clarkii, P. zonangulus) in Louisiana, USA

W. A. Baumgartner1,*, J. P. Hawke1, K. Bowles1, P. W. Varner2, K. W. Hasson2

1Louisiana State University School of Veterinary Medicine, Department of Pathobiological Sciences, Skip Bertman Drive, LSU, Baton Rouge, Louisiana 70803, USA
2Texas Veterinary Medical Diagnostic Laboratory, PO Drawer 3040, College Station, Texas 77841, USA

ABSTRACT: This is the first report of natural white spot syndrome virus (WSSV) infection in wild and large-scale farmed crawfish. In the spring of 2007, 3 crawfish farms experienced heavy mortality in ponds populated by Procambarus clarkii and P. zonangulus. Histological examination revealed findings consistent with severe viral infection characterized by numerous intranuclear inclusions in ectodermal and mesodermal tissues. Samples tested by in situ hybridization, injection bioassay in Litopenaeus vannamei, and PCR (nested and real time) were all positive for WSSV. Samples were sent to the National Veterinary Services Laboratory in Ames, Iowa, USA, where WSSV was verified. Subsequently, a multi-parish survey of 184 sites in Louisiana (including farm and wild basin samples) using real-time PCR determined that >60% of sites sampled were positive for WSSV, including wild basin samples.


KEY WORDS: White spot syndrome virus · WSSV · Crawfish · Crayfish · Procambarus clarkii · Procambarus zonangulus · Real-time PCR · Immunohistochemistry


Full text in pdf format  
Cite this article as: Baumgartner WA, Hawke JP, Bowles K, Varner PW, Hasson KW (2009) Primary diagnosis and surveillance of white spot syndrome virus in wild and farmed crawfish (Procambarus clarkii, P. zonangulus) in Louisiana, USA. Dis Aquat Org 85:15-22. https://doi.org/10.3354/dao02051

Export citation
Mail this link - Contents Mailing Lists - RSS
- -