DAO 85:115-122 (2009)  -  DOI: https://doi.org/10.3354/dao02076

Atypical furunculosis vaccines for Atlantic cod Gadhus morhua: impact of reattached Aeromonas salmonicida A-layer protein on vaccine efficacy

Vera Lund1,*, Helene Mikkelsen1, Merete B. Schrøder2

1Nofima Marin, Norwegian Institute of Food Fisheries and Aquaculture Research, PO Box 6122, 9291 Tromsø, Norway
2Norwegian College of Fishery Science, University of Tromsø, 9037 Tromsø, Norway

ABSTRACT: Atypical furunculosis caused by atypical Aeromonas salmonicida bacteria is reported as an increasing problem in farmed Atlantic cod Gadus morhua in Norway. At present, furunculosis vaccines adapted for cod or other marine fish species are not available. To identify bacterial components important for inducing protection in cod, we compared oil-adjuvanted vaccines based on A. salmonicida isolates phenotypically differing in their major cell surface constituents, such as the A-layer protein and lipopolysaccharide O-chains. Also included was an A-layer-deficient isolate with physically reattached A-layer protein. Vaccines containing A. salmonicida A-layer-producing cells elicited significantly better protection than vaccines with A-layer-deficient cells or with a supernatant with secreted A-layer protein. The A. salmonicida cells with reattached A-layer-protein resulted in significant and equal protection to the A-layer-producing cells and protected significantly better than the A-layer-deficient isolate. These results indicate that the A-layer protein when attached to the cell surface plays a role in inducing protective immunity in cod.


KEY WORDS: Atlantic cod · Aeromonas salmonicida · Furunculosis · Vaccine · A-layer protein


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Cite this article as:   Lund V, Mikkelsen H, Schrøder MB (2009) Atypical furunculosis vaccines for Atlantic cod Gadhus morhua: impact of reattached Aeromonas salmonicida A-layer protein on vaccine efficacy. Dis Aquat Org 85:115-122. https://doi.org/10.3354/dao02076

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