DAO 99:169-177 (2012)  -  DOI: https://doi.org/10.3354/dao02472

Isolation and characterisation of a novel Bohle-like virus from two frog species in the Darwin rural area, Australia

R. P. Weir1,*, N. J. G. Moody2, A. D. Hyatt2, S. Crameri2, R. Voysey2, J. Pallister2, I. V. Jerrett

1Department of Resources, PO Box 3000, Darwin, Northern Territory 0800, Australia
2Australian Animal Health Laboratory, Private Bag 24, Geelong, Victoria 3220, Australia

ABSTRACT: Twelve captive magnificent tree frogs Litoria splendida and 2 green tree frogs L. caerulea on a property in the Darwin rural area (Northern Territory, Australia) either died or were euthanased after becoming lethargic or developing skin lesions. Samples from both species of frog were submitted for histopathology and virus isolation. An irido-like virus was cultured from tissue samples taken from both species and was characterised using electron microscopy, restriction enzyme digests and nucleic acid amplification and sequencing. The isolates were determined to belong to the genus Ranavirus, were indistinguishable from each other and shared a 98.62% nucleotide similarity and a 97.32% deduced amino acid homology with the Bohle iridovirus over a 1161 bp region of the major capsid gene. This is the first isolation of a ranavirus from amphibians in the Northern Territory and the first report of natural infection in these 2 species of native frog. The virus is tentatively named Mahaffey Road virus (MHRV).


KEY WORDS: Ranavirus · Bohle virus · Iridovirus · Amphibians · Mahaffey Road virus · Litoria spp.


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Cite this article as: Weir RP, Moody NJG, Hyatt AD, Crameri S, Voysey R, Pallister J, Jerrett IV (2012) Isolation and characterisation of a novel Bohle-like virus from two frog species in the Darwin rural area, Australia. Dis Aquat Org 99:169-177. https://doi.org/10.3354/dao02472

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