MEPS 453:297-301 (2012)  -  doi:10.3354/meps09625

NOTE
Analyses of DNA obtained from shells and brine-preserved meat of the giant clam Tridacna maxima from the central Pacific Ocean

Jonathan P. A. Gardner1,*, Carolin Boesche1,2,3, Jan M. Meyer1,2, Ann R. Wood1

1Centre for Marine Environmental and Economic Research, School of Biological Sciences, Victoria University of Wellington, PO Box 600, Wellington 6140, New Zealand
2Department of Chemistry and Biology, University of Bremen, 28334 Bremen, Germany
3Present address: Carl von Ossietzky University of Oldenburg, 26111 Oldenburg, Germany
4Present address: Department of Chemistry and Biology, University of Bremen, 28334 Bremen, Germany

ABSTRACT: DNA was extracted from giant clam Tridacna maxima shells and from brine-preserved meats for sequencing of the cytochrome c oxidase I gene (COI). We examined COI variation in clams from Palmyra Atoll (Line Islands) and Tarawa (Kiribati) and incorporated our data into an existing phylogeographic analysis to identify one new central Pacific Ocean clade of clams. We highlight the utility of the DNA extraction procedures to address questions about illegal harvesting, sources of clams for the aquarium trade, and the origin of museum specimens.


KEY WORDS: Giant clams · Tridacna · DNA extraction · Phylogeography · Conservation


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Cite this article as: Gardner JPA, Boesche C, Meyer JM, Wood AR (2012) Analyses of DNA obtained from shells and brine-preserved meat of the giant clam Tridacna maxima from the central Pacific Ocean. Mar Ecol Prog Ser 453:297-301

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