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Diseases of Aquatic Organisms

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DAO 158:21-25 (2024)  -  DOI:

Epidemiological cut-off values for Yersina ruckeri disc diffusion data generated by a standardised method

Peter Smith1,*, Laëtitia Le Devendec2, Eric Jouy2, Emeline Larvor2, David Verner-Jeffreys3,4, Andrew Wokorac Joseph3,4, Elliot Stanton5, Edel Light6, Luana Cortinovis7, Tobia Pretto7, Amedeo Manfrin7, Pierre-Marie Boitard8, Matthieu Jamin8, Nicolas Keck9, Alain Le Breton10, Benoit Thuillier11, Christian Ravaille12, Sandrine Baron2

1School of Natural Science, University of Galway, Galway H91 TK33 , Ireland
2Anses, Ploufragan-Plouzané-Niort Laboratory, Mycoplasmology-Bacteriology-Antimicrobial Resistance Unit, 22440 Ploufragan, France
3Centre for Environment, Fisheries and Aquaculture Science, Weymouth DT4 8UB, UK
4UK FAO Reference Centre for AMR, Weymouth DT4 8UB, UK
5University of Bristol Veterinary School, Langford, Bristol BS40 5DU, UK
6Veterinary Medicines Directorate (VMD),Woodham Lane, New Haw, Surrey KT15 3LS, UK
7Fish Pathology Unit, Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy
8FILI@VET, 15 Rue du Puits, 29600 Saint Martin des Champs, France
9Laboratoire Départemental Vétérinaire de l’Hérault, 34000 Montpellier, France
10Selarl Vet’Eau, 31330 Grenade sur Garonne, France
11Labocéa, Microbiologie vétérinaire, site de Quimper, 29000 Quimper, France
12SOCSA Elevage, 81300 Graulhet, France
*Corresponding author:

ABSTRACT: In order to establish the meaning of data generated in antimicrobial agent susceptibility tests, it is necessary to develop internationally harmonised interpretive criteria. Currently, such criteria have not been developed for data generated in studies of the susceptibility of the fish pathogen Yersinia ruckeri. This work generated the data that would be required to set epidemiological cut-off values for the susceptibility data of this species that had been generated using a standardised disc diffusion method that specified the use of Mueller Hinton agar and incubation at 22°C for 24-28 h. Using this method, sets of inhibition zones data for 4 antimicrobial agents were generated by 3 independent laboratories. The data from these laboratories were aggregated and analysed using the statistically based normalised resistance interpretation. For ampicillin, florfenicol, oxytetracycline and trimethoprim-sulfamethoxazole the cut-off values calculated by this analysis were ≥16, ≥23, ≥24 and ≥30 mm, respectively. Evidence is presented demonstrating that the data for these 4 agents was of sufficient quantity and quality that they could be used by the relevant authorities to set internationally harmonised, consensus epidemiological cut-off values for Y. ruckeri.

KEY WORDS: CLSI VET04 · Normalized resistance interpretation · Antimicrobial susceptibility · Disc diffusion · Yersinia ruckeri

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Cite this article as: Smith P, Le Devendec L, Jouy E, Larvor E and others (2024) Epidemiological cut-off values for Yersina ruckeri disc diffusion data generated by a standardised method. Dis Aquat Org 158:21-25.

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