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Marine Ecology Progress Series

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MEPS 534:79-93 (2015)  -  DOI: https://doi.org/10.3354/meps11398

Contrasting transcriptome response to thermal stress in two key zooplankton species, Calanus finmarchicus and C. glacialis

Irina Smolina1,*, Spyros Kollias1, Eva F. Møller2, Penelope Lindeque3, Arvind Y. M. Sundaram4, Jorge M. O. Fernandes1, Galice Hoarau1

1Faculty of Biosciences and Aquaculture, University of Nordland, 8049 Bodø, Norway
2Department of Bioscience, Aarhus University, Frederiksborgvej 399, 4000 Roskilde, Denmark
3Plymouth Marine Laboratory, Prospect Place, West Hoe, Plymouth PL1 3DH, UK
4Norwegian Sequencing Centre, Department of Medical Genetics, Oslo University Hospital, Kirkeveien 166, 0407 Oslo, Norway
*Corresponding author:

ABSTRACT: Climate change has already led to the range expansion of warm-water plankton assemblages in the northeast Atlantic and the corresponding range contraction of colder-water species. The temperate copepod Calanus finmarchicus is predicted to shift farther northward into polar waters traditionally dominated by the arctic copepod C. glacialis. To identify temperature-mediated changes in gene expression that may be critical for the thermal acclimation and resilience of the 2 Calanus spp., we conducted a whole transcriptome profiling using RNA-seq on an Ion Torrent platform. Transcriptome responses of C. finmarchicus and C. glacialis from Disko Bay, west Greenland, were investigated under realistic thermal stresses (at +5, +10 and +15°C) for 4 h and 6 d. C. finmarchicus showed a strong response to temperature and duration of stress, involving up-regulation of genes related to protein folding, transcription, translation and metabolism. In sharp contrast, C. glacialis displayed only low-magnitude changes in gene expression in response to temperature and duration of stress. Differences in the thermal responses of the 2 species, particularly the lack of thermal stress response in C. glacialis, are in line with laboratory and field observations and suggest a vulnerability of C. glacialis to climate change.


KEY WORDS: RNA-seq · Copepods · Temperature stress response · Heat shock protein · Climate change


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Cite this article as: Smolina I, Kollias S, Møller EF, Lindeque P, Sundaram AYM, Fernandes JMO, Hoarau G (2015) Contrasting transcriptome response to thermal stress in two key zooplankton species, Calanus finmarchicus and C. glacialis. Mar Ecol Prog Ser 534:79-93. https://doi.org/10.3354/meps11398

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